Detection of Toxigenic Clostridium difficile : Comparison of the Cell Culture
نویسنده
چکیده
P. Pancholi*, C. Kelly, M. Raczkowski, J.M. Balada-Llasat 4 Department of Pathology. The Ohio State University, Columbus, OH 43205 5 6 7 *Please address all correspondence to: 8 Preeti Pancholi PhD. D(ABMM) 9 Department of Pathology 10 The Ohio State University Medical Center, 11 University Hospitals East 12 1492 East Broad Street 13 Columbus, OH 43205 14 Phone:614-257-3488 15 Fax: 614-257-2405 16 e-mail: [email protected]. 17 18
منابع مشابه
Molecular Analysis of Toxigenic Clostridium difficile Isolates from Hospital Environment by PCR Ribotyping Method
Background and Aims: Clostridium difficile is an identified cause of antibiotic-associated diarrhea, antibiotic-associated colitis, pseudomembranous colitis and nosocomial diarrhea. The objective of this survey was to determine molecular analysis of toxigenic Clostridium difficile isolates from hospital environment in Tehran tertiary medical centers. Materials and Methods: In this descriptiv...
متن کاملThe Frequency of Toxigenic Strains of Clostridium difficile in Hospitalized Patients with Diarrhea in Tehran/Iran by PCR Method, 2010
Background & Aims: Clostridium difficile has been identified as a pathogen in antibiotic associated diarrhea (AAD), pseudomembranous colitis and also nosocomial diarrhea. The present study was performed to find the prevalence of toxigenic strains of C .difficile isolated from diarrhea patients hospitalized in Tehran hospitals. Method: A total of 98 fecal samples obtained during July to December...
متن کاملToxigenic Clostridium difficile in retail packed chicken meat and broiler flocks in northeastern Iran
This study was designed to evaluate the occurrence of Clostridium difficile in both broiler chicken farms and packed chicken parts sold at market places in Mashhad, the second most popular Islamic pilgrimage city after Mecca in northeastern Iran. The fresh faecal samples were obtained from broiler farms, while the chicken packs were purchased from retail outlets across the city at market places...
متن کاملEvaluation of diagnostic tests for Clostridium difficile infection.
We evaluated toxigenic Clostridium difficile detection by a lateral flow assay for antigen and toxin, an enzyme immunoassay, and two commercial PCR methods. Compared to the cell cytotoxicity neutralization assay and toxigenic culture, both toxin detection methods lacked sensitivity. PCR following combined antigen and toxin detection provided the most useful diagnostic information.
متن کاملMulticenter clinical evaluation of the portrait toxigenic C. difficile assay for detection of toxigenic Clostridium difficile strains in clinical stool specimens.
We compared the Portrait Toxigenic C. difficile Assay, a new semiautomated sample-to-result molecular test, to a toxigenic bacterial culture/cell cytotoxin neutralization assay (TBC/CCNA) for the detection of toxigenic Clostridium difficile in 549 stool specimens. Stool specimens were also tested by one of three alternative FDA-cleared molecular tests for toxigenic C. difficile (Xpert C. diffic...
متن کاملDetection of Virulence Genes of Clostridium difficile in Children with Cancer by Multiplex PCR
Introduction: Toxigenic Clostridium difficile is the major cause of antibiotic-associated diarrhea, colitis, and pseudomembranous colitis. The pathogenicity of C. difficile is related to toxins A&B. Children with cancer are at risk of developing C. difficile infection (CDI) due to increased exposure to antibiotics, immunosuppression, and longer hospital stays. Recently, due to higher sensitivity...
متن کامل